Masturbation is perfectly healthy and it's a normal part of most people's lives. It's nothing to be ashamed of or to worry about and while it's not often talked about, most of Survey: How Often Do Americans Masturbate? Bespoke Surgical A frequently shared study from 2003 found men's testosterone levels increased around the seventh day of abstinence, with researchers observing a temporary spike of roughly 45 percent compared to baseline levels. However, What is the normal frequency of masturbation? ISSM